The phrase "generate a clone" is correct and usable in written English.
You can use it when referring to the production of a copy of something. For example: "Using advanced cloning technology, scientists were able to generate a clone of the rare species of frog."
In this approach, a library of bacterial artificial chromosome (BAC) clones is mapped onto chromosomes by the use of molecular markers, the aim being to generate a clone-based physical map with a "minimum tiling path".
Two years ago, company scientists had generated a clone of an endangered wild Asian ox, called a gaur.
The improvements of particular cloning steps cumulated to our optimized cloning pipeline thus generating a clone collection which covers > 83%% of the targeted ORFs (Table 4) thereby an efficiency > 90 was reached for ORFs up to 2 kb.
To establish the different 293T-REx lines, we first generated a clone harboring an inducible shRNA transgene against SCML2 (" shL2" clone); then, we transfected the different transgenic constructs (N3, N3 SCML2AWT and N3 SCML2AΔRBR) into the shL2 clone and new clones harboring both the inducible shRNA and the inducible SCML2 constructs were selected.
The final PCR product was gel purified and subsequently cloned into an Entry vector, pDONR221 (Invitrogen), to generate a master clone.
The 5' RACE clone with the longest 5' UTR region and the cDNA clone P21 were ligated together through a unique PmlI site at # 722 to generate a cDNA clone (Dazap1-C) with a near full-length insert.
To generate a sterile clone with fitness 2% greater than the population mean, a 1.5%-effect sterile mutation could occur in a population with less variation in fitness than is needed for a 0.6%-effect sterile mutation.
The PCR products of SAG5D gene was cloned into a pEASY-T1 vector (TransGen Biotech, China) to generate a recombinant cloning plasmid.
The purified PCR products were cloned by site-specific recombination into the donor vector pDONR221 to generate an entry clone.
