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They are involved in functions that are generally annotated as related to maintenance and repair as well as to some specific metabolic pathways (Fang et al. 2005).
Duplicated genes from teleost species are generally annotated as either gene-1/gene-2 or gene-A/gene-B according to the order of their discovery.
By searching for genes that are present in these four organisms (and that are generally annotated as methylansferase) we identified the COG2263 family.
We first identified a list of short (150 180 residues) proteins generally annotated as putative acetyltransferases and, in a second, more distant wave, the acetyltransferase domain of the canonical ArgA (NAGS) such as that of E. coli.
In both Methylocystis species, MbnS is followed by a gene resembling MoaA, a protein responsible for the first step in molybdenum cofactor biosynthesis [ 45] (which involves the conversion of a guanosine derivative to precursor Z) and a gene generally annotated as a 3-hydroxyisobutyrate dehydrogenase.
These larger TBDTs are preceded by an ORF generally annotated as an "Fe(III) dicitrate membrane sensor" (PFAM PF04773) and an "ECF sigma factor" (with conserved σ-70-like regions 2 (PFAM PF04542) and 4 (PFAM PF08281)), designated MbnR and MbnI, respectively.
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Transcripts annotated as known transcripts are generally those for whom a full length cDNA exists.
Contigs were annotated as above.
All sequences were annotated as above.
Metabolites in early reconstructions were generally annotated manually.
Metabolites are generally annotated with various database or structure based identifiers.
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