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A few individuals of three additional species (Quercus bicolor, Betula nigra, Platanus occidentalis) were monitored over a shorter time period to assess the generality of responses.
Species comparisons enhance our ability to test the generality of responses to challenges, apply ecological theory to the explanation of behavior, and incorporate the feeding strategy of a species in the study of its ingestive behavior.
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Additional research will be necessary to determine the generality of these responses and their underlying mechanisms.
To assess further the generality of these responses, we plated the isolated HASM cell on substrates of different stiffness.
To assess further the generality of these responses, we subjected the cell to a transient stretch of longer duration (30 seconds, Figure S1c).
However, the generality of such responses in all long-lived species is questionable; some breeding seabirds will, to a limited degree, adjust foraging patterns to changes in environmental conditions at the expense of their future survival [15], [16].
Experimentally imposed decreases in activity in cultured hippocampal cells tend to lead to generally similar alterations as in cultures of the visual cortex, including neuronal hyperexcitability, increased glutamatergic transmission and decreased GABAergic synaptic inputs [4], [7], [16], [19], [21], [22], indicating the generality of homeostatic responses.
The generality of stress responses at the transcriptional level was therefore time dependent.
To test the generality of eavesdropping responses, we conducted two additional one-pheromone trials per colony using LGE from bees captured from a different location (approximately 500 1,000 m away).
Identical experiments were performed in human 293 cells to determine the generality of this response.
To test the generality of this response, we used the same cell populations and induced cardiac differentiation by the hanging drop method.
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