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The pipette was positioned 100 µm away from the axonal growth cone at a 45° angle from the original direction of axon outgrowth, which was defined by the direction of the 20 µm axon segment; 2 Hz pulses (3psi, 20ms) were generated with a pulse generator (Grass Product Group, SD9) and a Picospritzer II (General Valve).
In general, valve technology is mature, but there are still developments in materials, design, and automation to be made.
We describe our experiences with in particular the General Valve (GV), Jordan Valve (JV), and Nijmegen Pulsed Valve (NPV) as a source for Stark deceleration experiments.
Frequently used valves include the piezo activated valve [29], General Valve, Jordan Valve, Even-Lavie Valve [30], and more recently the Nijmegen Pulsed Valve [31].
In the studies performed to date an Even-Lavie valve (ELV) was used for one expansion while a General valve (series 99; GV) was used for the other one.
The virus (1 2 µl into a single injection site) was delivered with a glass micropipette by pressure injection (20 psi, controlled by a Picospritzer II, General Valve, Fairfield, NJ, USA).
Similar(50)
The design of the HSC was similar to a general spool valve comprising a spool and a steel housing.
I think it would be helpful for the authors to highlight better which are the issues that are truly specific to these patients (i.e. retroperitoneal hematoma, and possible management of severe COPD) and which are general to valve surgery (e.g. risk of stroke).
It is a general-service valve that is used primarily for on-off, non-throttling applications.
In general, mechanical valves are used in the younger age group and require lifelong anticoagulation.
In general, cryopreserved homograft valves function well in the right ventricular outflow tract.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com