Extensive study of Plasmodium species and T. gondii has established that proteases help to coordinate and regulate the lifecycles of these parasites, playing key roles in host cell invasion, general catabolism, host cell remodelling and egress from host cells [ 1].
This is illustrated by a mutant generated using dithitotreitol as an egress enhancer, which displays a defect in dithitotreitol induced egress but not in Ca2+ ionophore induced egress.
Three general approaches have been taken to establish the identity of proteases participating in egress: analysis of the subcellular localization and substrate diversity of known malarial proteases; gene disruption studies; and the use of selective small compound inhibitors.
Studied already in great biological detail, herpesvirus nuclear egress therefore represents a unique tractable model system to delineate the general structural and functional basis of nucleo-cytoplasmic vesicle transport.
Finding a coat of TorsinA-LAP1 complexes, or any other players implicated in nuclear egress, might then suggest a similar mechanism of vesicle formation in a general nucleo-cytoplasmic transport pathway of large cargo, as described here for nuclear egress of herpesviral capsids.
Nevertheless, such roles, if any, can only be auxiliary, because parasites that lack SERA1 and SERA2 display no defects in egress of liver and blood stage merozoites, or natural malaria transmission in general.
Living spaces have egress windows.
They do not have proper egress.
It has an egress from Central Park.
Barnum hastily made sign saying "This way to the egress".
