However, this regulation of MBF is not a general checkpoint response, because cdc22 is not up regulated by activation of the G2 DNA damage checkpoint.
Therefore, NFATc2 is a general checkpoint, controlling the participation of activated Th cells in an immune reaction in a binary fashion.
Besides being involved in general checkpoint responses like inhibition of mitosis and activation of DNA repair mechanisms, the DNA replication checkpoint is specifically aimed at stabilizing stressed replication forks 92, 93.
In general, the checkpoint arrests cells in mitosis until all chromosomes have aligned at the metaphase plate.
Our results suggest that this might be similar for Plk1 mediated mitotic arrests, suggesting that this is a general spindle checkpoint associated phenomenon.
It has been proposed that the G2 delay induced by chromosome entanglements is controlled by a more general antephase checkpoint that responds to aberrant chromatin topology and is mediated by p38 MAP kinase (Mikhailov et al, 2004).
But Bob Butterworth, Florida's attorney general, called the checkpoints "inexcusable" and "flat-out wrong," even though an investigation by his office found no evidence that voters were intimidated.
Russell Holland, UK general manager of Checkpoint Systems, said: "Over the last year, retailers have certainly been making great strides in the fight against retail crime.
These results demonstrate the general importance of checkpoint regulation of G1/S transcription in response to replicative stress and elucidate the specific roles of Mik1 and Mrc1 in the checkpoint.
However, in our experimental settings, we did not observe defects in the recruitment of DNA damage signaling proteins in general or in checkpoint activation following Ago2 or Dicer depletion (Supplementary information, Figures S2, S3 and S4A, S4C-S4F).
