Indeed, as described in Results, 30 pM nocodazole did not result in overt perturbations in mitotic morphology, mitotic progression, and general cell and tissue features.
We conclude that topoisomerase IIalpha is a strict proliferation marker in normal and neoplastic cells in vivo, but that topoisomerase IIbeta has a much more general cell and tissue distribution than has topoisomerase IIalpha.
Further analyses of various subclasses of the invariant units suggest that the degree of correlation is strongest for the general-responsive units in comparison to the sub-general cells and the specific neurons (Figure 17D F); Again, this is in line with the results from the individual data.
Thyroid tissue consists of many different cell types, both tissue-specific (thyrocytes and C cells) and general cells (e.g., endothelial, muscular, and fat cells).
On the contrary, COX-2 disruption in BMSC cultures decreased osteoblastogenesis, and general cell growth and inhibit or delay fracture healing [ 45- 47].
Focusing on the genes differentially regulated by E2 in ERβ- vs ERβ+ cells known for their involvement in cell proliferation, we observed that many of them encode for transcription factors and other key proteins controlling large gene networks of cell division cycle and cell survival and, in general, cell proliferation and differentiation pathways.
The slices were stained with hematoxylin and eosin for general cell morphology and identification of cartilage tissue, and visually inspected using light microscopy.
This aspect is of general cell biological and biochemical interest and deserves further studies.
It is important to note that the differentiation markers assessed in the above studies are bone- and fat-specific, and do not simply reflect general cell viability and health.
We favor a model in which protein DX causes the cell to behave as if it were nutrient depleted and react by lowering general cell metabolism and increasing secondary ATP synthesis pathways.
