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Indeed, as described in Results, 30 pM nocodazole did not result in overt perturbations in mitotic morphology, mitotic progression, and general cell and tissue features.
We conclude that topoisomerase IIalpha is a strict proliferation marker in normal and neoplastic cells in vivo, but that topoisomerase IIbeta has a much more general cell and tissue distribution than has topoisomerase IIalpha.
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They were sent to our members in the general cells and rejected there too.
On the contrary, COX-2 disruption in BMSC cultures decreased osteoblastogenesis, and general cell growth and inhibit or delay fracture healing [ 45- 47].
The slices were stained with hematoxylin and eosin for general cell morphology and identification of cartilage tissue, and visually inspected using light microscopy.
This aspect is of general cell biological and biochemical interest and deserves further studies.
It is important to note that the differentiation markers assessed in the above studies are bone- and fat-specific, and do not simply reflect general cell viability and health.
We favor a model in which protein DX causes the cell to behave as if it were nutrient depleted and react by lowering general cell metabolism and increasing secondary ATP synthesis pathways.
For general cell lysis and co-immunoprecipitation of Flag-X and the candidate interactor Myc-Y, HEK293 cells were transfected with indicated expression vectors by Lipofectamine 2000.
EGFR and its downstream kinase cascades play essential roles in general cell growth and differentiation.
Genes with increased expression in improvers post-treatment included genes associated with general cell growth and cell cycle-related processes such as DNA repair (POLE, SWI5 and RAD52), microtubule cytoskeleton (DOCK7) and mRNA processing (CDK12).
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