Therefore, the observation that vitrification is not critical for cryopreservation in the studied Angraecum and Benthamia species from Madagascar has significance to the conservation of other related species from the genera and beyond.
Although detailed analysis of the morphological data for all genera is beyond the scope of this study, we include the full morphological data matrix in Additional file 1.
A complete characterization of Blochmannia in these genera is beyond the scope of the current study, but ideally will include additional representatives of each genus to test for symbiont persistence and stability, and will be coupled with ultrastructural work to document the location of bacteria within host tissues or cells.
Regrettably, we have no experimental access to many of the GenBank accessions, and a taxonomic revision of the two genera is far beyond the scope of the present study.
One R.PabI and M.PabI homolog gene pair is observed immediately after the GMP synthase gene in both Campylobacter and Helicobacter, representing orthologs beyond genera.
A natural corollary is that in conducting global biogeographic analyses it may often be necessary to move beyond traditionally recognized genera and families, and gravitate instead to much larger, more inclusive clades, most of which will not have been formally named.
Second, isolates from only 3 genera were included, limiting the generalizability of any conclusions beyond these organisms.
Third, all the assessments we made were in analyses that focused on individual genera, because it was not possible to unambiguously align non-coding regions beyond the genus level.
Ortho-proteogenomic analyses have, to date, been limited to only two genera, Mycobacterium [ 19] and Yersinia [ 20], and have not been extended beyond this taxonomic level.
Our comparative survey focused on individual genera, because we were generally not comfortable aligning most of the non-coding regions beyond the level of genus.
