All bacterial sequences were classified at the phylum level down to the genus level using the Mothur program, and the relative abundances of the assigned phyla and genera were compared between the two samples.
The arrangement among the genera, however, varied between the two studies.
In a 4-way comparison of orthologs in C. elegans, C. briggsae, A. caninum, and A. ceylanicum, the phylogenetic distance between A. caninum and A. ceylanicum is almost identical to that between C. elegans and C. briggsae and the distance between the two genera is just over four times the within genera distance.
For example, if genus Pseudomonas had the genus Cellvibrio as its closest partner in 100 trees, then k = 20% would require other candidate genera to be partnered with Pseudomonas in at least 20 other trees to generate a link between the two genera in the IAG.
A better understanding of the phylogenetic relationship and divergence time of species within the Anopheles and Aedes genera will also be helpful to determining the timing and direction of the horizontal transfer of MJ1 between the two genera.
Due to the ambiguous phylogenetic relationship between the two genera [ 47, 56], we furthermore added the following closely related Asian viviparid genera to our dataset: Anulotaia, Idiopoma, and Trochotaia.
In the present study, four gene loci 16S rRNA, ITS-L, cpcBA-IGS and rpoC1, sequenced from many Chinese strains (44 Cylindrospermopsis strains and 16 Raphidiopsis strains), were analyzed to infer the phylogenetic relationship between the two genera.
This difference in evolutionary divergence between the two genera may be due to differences in their activity patterns.
