Gene amplification means the repeated replication of a certain gene without a proportional increase in the copy number of other genes and is a widespread phenomenon in eukaryotes.
Gene gain was considered based on the copy number for that gene, without regard for entire chromosome gain or loss.
PsAvr3c gene without the signal peptide was cloned into the yeast vector pGBKT7 (Clontech).
"First, changing histones can change the activity of a gene without affecting the sequence of the DNA".
Biologists call this gene imprinting an epigenetic, or "on-genetic," effect, meaning that it changes the behavior of the gene without altering its chemical composition.
"Handedness is a complex behavior," Dr. Geschwind said, "and no complex behavior has ever been shown to be due to only a single gene without any environmental influence".
The streptokinase gene without its native signal peptide was cloned and expressed in Escherichia coli, using the pRSETB expression vector.
Furthermore, HRP C1a was secreted into the culture medium from cells expressing the HRP C1a gene without the CTPP region.
For the most part, though, it's nearly impossible to turn off a gene without deleting it (which means you can't turn it on again).
The paradox is that now that we've invented techniques that introduce just one gene without disturbing the rest, some people think that's terrible.
Knock down of endogenous Tia1 increased expression of the renilla luciferase reporter gene without changes in the abundance of renilla luciferase mRNA (Fig. 5b and Supplementary Fig. 5D).
