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The genome of each metazoan species encode only a single DIA1 gene, with the exception of the zebrafish (Danio rerio) and minnow (Pimephales promelas) genomes, which encode two closely related DIA1 genes (Tables 1, S1 and S2).
The PCR product for each CDS corresponded to the full length of the putative gene, with the exception of genes longer than 3 kilobases (kb) that were split into two or three PCR products.
However in some cases the pattern of distribution does appear unusual: in a number of Molluscan and Annelide sequences, most introns are concentrated in the 5' part of the gene, with the exception of a single 3' one.
All parasite species have one SERA gene from Group I, Group II and Group III (cysteine-type SERA gene), with the exception of P. gallinaceum, which has only two SERA genes, one from Group I and the other from a common ancestor of Groups II and III (Figure 2).
All genomes encoding a DIA1R gene also had a DIA1 gene, with the exception of the EST databases for cartilaginous fish species and a single ostariophysan species (Ictalurus punctatus), where partial DIA1R sequences (Table S6), but not DIA1 sequence(s) was detected.
The eukaryotes and the Euryarchaeota contain only one PCNA gene, with the exception of a few near identical copies of unknown functionality in Drosphila, Arabidopsis, and Thermococcus (see Figure S1) that are generally not present in closely related taxa (data not shown).
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Indeed the autotrophic arsenite oxidase genes form a distinct phylogenetic group, separated from previously described heterotrophic arsenite oxidase genes, with the exception of the heterotroph Agrobacterium tumefaciens.
All of the selected genes, with the exception of four genes, were expressed in at least one of the tissues tested, which indicates that the NAC genes are involved in various aspects of the physiological and developmental processes of the apple.
All these resistance genes, with the exception of the tetA gene, were also found on the pEC_L46 plasmid backbone.
None of the above mentioned genes, with the exception of SFTPA1, have been previously correlated with disease progression in IPF.
The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone.
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