Sentence examples for gene was digested from inspiring English sources

The resulting PCR product, lacking most of the coding sequence of the gene, was digested with XbaI enzyme and ligated into similarly a digested pCVD442 suicide plasmid.

The PCR fragment containing the FP gene was digested with HindIII/KpnI and subcloned instead of GFP in the digested GA pcDNA3 vector.

The lpp-ompA fusion gene was digested from plasmid pGEM-JR with NdeI and HindIII and then ligated into similarly digested pET-26a to generate pLpp-OmpA.

To determine the spatial expression of dep, the promoter region (including an 80-bp proximal region) of the gene was digested with the appropriate enzymes and fused to the GUS coding sequence via ligation to the pBl101 plasmid vector.

The PCR fragment of KanR gene was digested with Avr II/Sac II and ligated to Nhe I/Sac II-digested pGEM-pSa vector to generate pGEM-pSa-Kan.

(A) The T vector with HEV-ORF2 gene was digested with NruI and XbaI, and the resultant 2-kb fragment was ligated with a transfer vector pVL1393 digested with SmaI and XbaI.

The pHT01 plasmid and the amplified kIspS gene were digested using BamHI and XbaI restriction enzymes.

The fragment of SPT15 mutant gene and pYX212 plasmid were digested with EcoRI and SalI, however the pYX212 plasmid and TAF23 gene were digested by using restriction enzymes SalI/NheI and ligated by using T4 DNA Ligase (Thermo Scientific CO., LTD, Meridian, USA) to get pYX212-kan-SPT15-Mutant (pYX212-kan-SPT15-Mu) and pYX212-kan-TAF23-Mutant (pYX212-kan-TAF23-Mu) plasmids, respectively.

The pGEM-T Easy Vector containing YopP and the amplified kanamycin resistance gene were digested with SalI (NEB, Ipswich, MA) and SacI (Fermentas, Glen Burnie, MD) and ligated.

The vectors containing native or modified versions of the PHO8 gene were digested with the AhdI.

The amplified PCR products of hsp60 gene were digested by Hind III enzyme.