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A 3,680-nt 3,680-nts full sequenconsensus spike (S) gene was completed by classical PCR and Sanger sequencing (GenBank/EMBL accession no. Hfull506).
An F-test for gene was completed using the mean square for gene divided by the mean square for amplicon within gene to determine if gene effects were significant above those detected for amplicon within gene.
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By elimination of unconnected-reactions from BsRN-2016, reconstruction process of the first third-generation GEM iBsu1144 employing 1083 reactions linked to 719 genes was completed.
In this work, real-time PCR amplification of blaCTX-M, blaSHV and blaTEM genes was completed by multiplex pyrosequencing characterization of blaTEM and blaSHV in order to detect ESBL associated variants in isolates from Bulgaria, Romania and Democratic Republic of Congo.
Identification of 40,000 cane genes was completed in 2003 and there are a couple dozen research groups working on the functional genome, still on the experimental phase, but commercial results are expected within five years.
RT-PCR amplification of alternative pre-mRNA splicing isoforms derived from mini-genes was completed as previously described.
Sanger sequencing of the hemagglutinin and neuraminidase genes was completed, and consensus was used to construct a Kimura 2-parameter neighbor-joining tree (Technical Appendix Figure 1).
The mapping between microRNAs and its host genes was completed using a simple method that utilizes genomic coordinates retrieved from miRBase [ 23].
To make sense of the large amount of transcriptome information obtained, functional and pathway classification of significantly altered genes was completed.
Sequencing and analysis of the 4.6 Mb chromosome of the laboratory strain E. coli K12 coding for 4411 protein-coding genes was completed in 1997 [ 1].
Identification of orthology of the DE and candidate genes was completed as follows: the Midas contig was blasted in Ensembl against medaka or zebrafish and the longest CDS was obtained.
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