Gene dosage of each target and reference gene was calculated using the standard curve method.
The coverage of each gene was calculated with 'BEDtools coverage' in BEDtools suite version 2.17.075.
Total expression of the gene was calculated for each cell type along with the percentage of each splice variant.
The relative expression of each gene was calculated by the Pfaffl method (Pfaffl, 2001) implemented in the Eco qPCR system software.
The relative quantity of amplified cDNA corresponding to each gene was calculated by using the ΔΔCt method and normalized for expression of gapdh in each sample.
Multiple nucleotide sequence alignment of gene was calculated and presented by using the DNAMAN program.
A The RPKM value for each gene was calculated using uniquely mapped reads.
At least three replicates were performed, and the mean expression level for each gene was calculated.
The signal intensity of each gene was calculated as follows: (Row signal intensity/75th percentile of each array) × 2,725.
The relative fold change for each gene was calculated using 2−ΔΔCT method.
The number of GC/µl of the stock prepared for each gene was calculated.
