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Labelling was performed using retroviral gene transduction.
Aoki, T. et al. Improvement of lentiviral vector-mediated gene transduction by genetic engineering of the structural protein Pr55 Gag.
Baculovirus mediated gene transduction of mammalian cells (BacMam) is an emerging technique for rapid recombinant protein expression in mammalian cells.
In the improved protocol, gene transduction efficiency to murine T cells was more than 90%.
HIV-1 derived vectors are among the most efficient for gene transduction in mammalian tissues.
In addition, activation of P2X7R by BzATP or overexpression of P2X7R via gene transduction reversed the inflammation-mediated decrease in PDLSC osteogenic differentiation.
This method has an advantage compared with Wnt activators since iTSCM cells can be converted from antigen-specific memory T cells, thus TCR gene transduction is not necessary.
Furthermore, using ex vivo gene transduction, we evaluated the potential for ectopic bone formation by the transduced MSCs in vivo.
HGF gene transduction inhibited fibrogenesis and hepatocyte apoptosis and also produced resolution of fibrosis in the cirrhotic liver.
In a comparison of gene transduction efficiency, the LpDNA system showed better cell transduction than the pDNA system.
To determine the effect of P2X7R gene transduction on osteogenesis under inflammatory conditions, we evaluated the osteogenesis of gene-modified cells in response to exposure to inflammatory factors or a P2X7R antagonist (BBG).
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