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The figure was constructed using Biovenn bioinformatics tool.59 (c) Gene term heatmaps generated in the DAVID analysis tool from the top 300-ranked target genes.
Positive gene term associations are represented in green, and currently unreported gene term associations are represented in black.
Of these tasks, the gene term recognition and normalization were the most difficult.
All gene term curation was performed prior to testing for associations.
These terms are variations of the original gene term but prefixing the letter h to indicate that it is a human gene.
The Database for Annotation, Visualization and Integrated Discovery (DAVID) [ 60, 61] identifies if a given list of genes is significantly enriched in an annotated gene term.
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Removal of false positives (FPs) uses information in the gene name itself, the surrounding text, as well as entire paragraphs or full text to ensure that a found name refers to a specific gene, and not another non-gene term.
Similarly, ISS-tagged (Inferred from Sequence Similarity) gene-term associations in Gene Ontology were also excluded from the training and test sets.
Thresholds for Gene count and EASE score, which provides the significance of gene-term enrichment with a modified Fisher's exact test, were set equal to 2 and 0.05, respectively.
GO terms and gene-term associations were downloaded from the February 2009 snapshot of Gene Ontology database [38] (www.geneontology.org).org
In the functional annotation analysis, modified Fisher's exact test was used to determine the significance of gene-term enrichment with a cutoff value at P = 0.05.
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CEO of Professional Science Editing for Scientists @ prosciediting.com