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Sequence analysis of ITS domain and/or D1/D2 domain of the 26S rRNA gene techniques were applied in identifying yeasts for various purposes.
Coupled with materials science, cell biology, biochemistry, and gene techniques, some novel approaches have been developed to overcome the current challenges such as enhancing angiogenesis, inhibiting scarring, and regenerating appendages.
DOI: http://dx.doi.org/10.7554/eLife.06394.006 Using single gene techniques, we previously found that Hairy induces a widespread histone H4 deacetylation throughout the entire ftz locus (Li and Arnosti, 2011).
We searched expressed sequence tag (EST) databases for novel TCF7L2 transcripts and sought to validate the function and integrity of any isoforms found using a combination of RT-PCR, western blotting and reporter gene techniques.
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This can be measured by a reporter gene technique.
The amplification of 16S rRNA gene technique is based on amplification of hypervariable regions of the gene anchoring to conserved sequences.
Inteins have been utilized in the production of protein and peptide arrays, as molecular switches and in the reconstitution of functional proteins by split-gene techniques.
Experiments exploiting older technologies, like those used by Subramanian et al. [ 19] hold the middle ground between traditional single-gene techniques and high-throughput implementations and cannot provide sufficient data for systemic interpretation.
But some scientists say the best chance for success now lies in using modern gene-sequencing and gene-mapping techniques to find the gene or genes responsible for apomixis, and then transferring them to crops.
Scientists called a voluntary moratorium on gene recombinant techniques (rRNA) and convened the Asilomar conference to discuss the risks inherent in recombinant techniques.
There are two basic types: gene silencing techniques such as RNA interference (RNAi) that can turn particular genes off; and gene editing techniques such as CRISPR, TALEN or zinc fingered nucleuses used to cut DNA in order to make small genetic changes or insert genetic material.
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