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In the 1930s a German geneticist claimed to have found a gene shared by many male homosexuals.
The protein sequences surrounding this gene shared high similarity with those surrounding chicken GRIN2A gene.
If more than one gene shared identity at >90%, the higher value was kept.
Overall DNA comparison of the asdA gene showed that the E. ictaluri asdA gene shared 72% identity with the Escherichia coli asdA gene.
Remarkably, there is not a single gene shared by the 7 signatures tested here (the 6 best performing previously reported signatures and the 17 probe set signature derived in this paper).
While the different copies of one family were ∼85% identical at the nucleotide level, this gene shared 75% nucleotide identity (134/177) and 61% amino acid identity (48/78) with the flanking genes.
Furthermore, a chicken GRIN2C gene was found using the similar method in Chromosome 18, and the protein sequences surrounding this gene shared high similarity with those surrounding chicken GRIN2A gene (data not shown).
Probe-sets detecting exclusively transcripts carrying a miR-seed target are more strongly affected by miRNA regulation than probe-sets that detect a region of the gene shared by transcripts that do not carry the miR target sequence.
Two euchromatic genes LIPI and SAMSN1 were excluded from the methylation analysis because the former gene shared a CpG island with RBM11 (the two genes are head-to-head with the CpG island in the middle) and the latter gene had no associated CpG island.
An alternative explanation might be that this 7-nt deletion and the 4 other mutations found in the pyrE2 gene – shared by 42 of the mutants - were a pre-existing set of mutations present in the initial cultures used for the fluctuation tests, which were a subset of the 6 fluctuation tests performed to calculate the genomic mutation rate of H. salinarum NRC-1.
This gene must have been acquired much more recently than the gene shared by most Agaricomycotina.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com