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For each organism to be typed, the gene sequences were extracted from the genomic record using the GenBank annotation.
The conserved core regions of the coat protein gene sequences were extracted from the genome dataset alignment and used to calculate a neighbor-joining tree using the program MEGA3 [20]; the core regions were those bounded by the sequences encoding the WCIEN and QMKAAA motifs.
Gene sequences were extracted from Hmel1.1 and annotated using FlyBase and GO Elite.
The rp gene sequences were extracted from three database sources [see Additional file 2].
Tetraodon and stickleback TAAR gene sequences were extracted from the ENSEMBL database and curated by hand (this work).
Gene sequences were extracted from the soybean reference genome which was generated from the cultivar Williams82 [ 23].
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The corresponding gene promoter sequences were extracted from UCSC database (mm6) [ 17].
For the KLF family of genes in Table 4, uAUG sequences were extracted from the 5'-UTR portions of the full RefSeq mRNA.
To calculate the percent identity of 16S genes between genomes, 16S rDNA sequences were extracted from the noncoding RNA file for each genome (".frn"), and the pairwise sequence identities were calculated using USEARCH global alignments (Edgar 2010).
Candidate sequences were extracted from the 50 most highly upregulated genes from the microarray analyses.
Only continuous sequences were extracted from genomes.
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