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Although it was beyond the scope of this study to test ST75/ST883 for bacterial interference with other S. aureus strains or to determine the amino acid sequence of the mature AIP from ST75 and ST883, predicted AIP amino acid sequences derived from gene sequences were analysed.
When putative wheat gene sequences were analysed for ORF length based on their hit status, we observed significantly shorter ORFs in sequences with no hits.
All 16 rRNA gene sequences were analysed with Chromas software (Griffith University, Queensland, Australia) and aligned using Clustal X software [ 42].
The predicted gene sequences were analysed for the presence of a leader peptide using SignalP 3.0 [ 40] and transmembrane regions using the TMHMM server 2.0 http://www.cbs.dtu.dk/services/TMHMM-2.0.
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The 16S rRNA gene sequence was analysed through NCBI BLAST (http://www.ncbi.nlm.nih.gov/blast/) and EzTaxon servers (http://www.eztaxon.org/).org/
Based on these morphological and biochemical characteristics, the strain OP1 is homologous with Bacillus sp. The 16S rRNA gene sequence was analysed with the Robust Phylogenetic tree online tool [30, 31], and the neighbour-joining dendrogram was constructed (Fig. 1).
Mouse and rat PSG gene coding sequences were analysed using an NJ plot which highlighted four putative orthologous relationships, as follows: rat PSG36 and mouse Psg24; rat PSG40 and mouse Psg29; rat PSG42 and mouse Psg32; rat PSG38 and mouse Psg16 (Fig. 6).
In order to establish the nature of the variants located in the introns of the ABCA4 gene, wild type and mutated sequences were analysed with Prediction Servers of Intron Splice Sites in human (Center for Biological Sequence Analysis of University of Denmark (http://www.cbs.dtu.dk/services)).dtu.dk/services
Both contig and SNP allele sequences were analysed for gene content by blastx using the ENSEMBL non redundant protein databases for zebrafish (Danio_rerio.Zv9.64.pep.all.fa).fa
Once the open reading frames of relevant gene entries were established, the resulting protein sequences were analysed in various ways.
Sequences were analysed using Seqscape v2.5 software.
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