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The gene sequence alignment of flavin nucleotide binding domain indicates that this is a highly conserved domain across many azoreductases.
The test for positive selection was conducted using a likelihood-based method, where the likelihood of different evolutionary models is computed given a gene sequence alignment and a reconstruction of the phylogenetic history of the genes.
Additional file 3: TAAR gene sequence alignment.
File_3 includes each gene sequence alignment of coding region among the three species with guinea pig.
16S rDNA gene sequence alignment has been used as the reference method for mycobacterial species identification.
(ZIP 342 kb) Additional file 3: File_1 and File_2 include each gene sequence alignment of non-coding and coding region among T.
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Given the high degree of sequence similarity between some of the stefin members (Figure 2A), gene sequence alignments were required to design SYBR green-based qRT-PCR assays specific to Stfa1 (detecting also BC117090 and BC100530), Stfa2, Stfa2l1, and Stfa3 (Text S1 and Table S3).
Even when one has high quality rRNA gene sequence alignments, phylogenetic analysis involving very deep branches in the tree of life can still be difficult due to inherent complications, such as convergent evolution due to GC content effects [40], [41], [42].
While this constraint was not an issue for our longer gene sequence alignments, it caused substantial problems for more than half of the shortest gene sequence alignments.
We re-estimated the gene trees using RAxML on the gene sequence alignments produced by Song et al. (2012).
Previous studies have only examined coalescent-based methods on gene sequence alignments with at least 100 sites.
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