Sentence examples for gene requirements from inspiring English sources

To investigate essential gene requirements in model organisms, multiple approaches have been used to reduce gene function and infer gene requirements based on the resulting mutant phenotypes.

To study essential maternal gene requirements in the early C. elegans embryo, we have screened for temperature-sensitive, embryonic lethal mutations in an effort to bypass essential zygotic requirements for such genes during larval and adult germline development.

To further promote the use and isolation of conditional mutations in essential C. elegans genes, we have surveyed this collection of new conditional mutants for essential gene requirements during both larval and early embryonic development, and we have determined whether all are fast or slow acting.

Finally, for genes that are expressed both maternally and zygotically, maternal expression of a wild-type allele can in some cases compensate for lack of zygotic expression in homozygous mutant progeny, precluding the identification of some gene requirements early in development when non-conditional alleles result in lethality due to later essential zygotic requirements.

Particularly with fast-acting TS mutations, one can identify multiple essential requirements, and define temperature-sensitive periods of gene requirements, sometimes even within a single cell cycle, or during the entire life span of an organism, by performing temperature up-shifts and down-shifts at different times [8], [9].

We performed a genome-wide study of essential and nonessential S. cerevisiae gene requirements during grape juice fermentation to identify deletion strains that are either depleted or enriched within the viable fermentative population.

This infection site-specific gene requirement was verified by targeted mutagenesis in the selected genes.

Therefore, RNA silencing using specific antisense sequences provides an approach to measure gene requirement stringency.

Therefore RNA silencing can be used to confirm and quantify essential gene requirement and serve as a molecular pre-screen, before investing in a search for specific inhibitors.

Nevertheless, correlation between mRNA and protein levels in bacteria has been demonstrated for some genes [25], [39], [45], [46], [47], suggesting that the MTL values reported here provide a useful measure of gene requirement.

This condition provides a stringent assay for downstream gene requirement.