BS carried out the 16S rRNA gene library preparation, constructed sequence contigs and performed phylogenetic identification.
Author 3, Husebye, H., participated in cloning of cDNA and gene, and preparation of the manuscript.
Microarray gene expression preparation and processing procedure were done as recommended by the manufacturer (Affymetrix, Inc .. Raw expression values were extracted and normalised by the Affymetrix Power Tools based on Robust Multiarray Average (RMA) approach.
We assembled Illumina reads, identified and extracted plastid genes, and concatenated and aligned genes in preparation for phylogenetic analysis as follows.
If the samples contained extensive normal lesions, we excluded such samples by the expression profile-based scoring method using normal and/or tumor specific genes (in preparation).
TT and AS-M designed and performed experiments with ethylene related genes and preparation of the manuscript.
It is plausible that under furfural stress, C. beijerinckii 8052 may sense sulfur limitation and consequently elevate expression of sulfate ABC transporter genes in preparation for potential increased absorption of sulfur from the fermentation medium.
These can be used as positive controls by spiking in a 'cocktail' of the corresponding bacterial mRNAs in known quantities (for details on control genes and preparation of mRNA 'cocktails' by in vitro transcription, see [ 36]).
Prl regulates the protein expression of NF1-C2 iNmuMGMG cells, and its expression is reduced in the nucleus of Prlr-/ luminal cells at mid-pregnancy, indicating that NF1-C2 may be regulated by Prl signalling during pregnancy and involved in expression of milk genes in preparation for lactation [ 62].
Expression of selected adipose genes in preparations of bone marrow cells, adipocytes isolated from bone marrow and adipocytes isolated from epididymal white adipose tissue.
