Thus, we make available the list of 893 putative synaptic genes to the scientific community, firmly believing that this will facilitate their investigation, by means of gene silencing, mutant analysis, behavioral assays and other traditional protocols.
Examining differential expression in the NSF45K and other array data pointed toward another candidate gene, which has been supported by mutant analysis of gene 5-1.
The genes in this cluster (Additional file 9: Table S2) included genes that in our mutant analysis were important in lesion formation, including SID2, PAD4, WRKY25 and WRKY33.
Another 41% (286 genes) were identified by mutant analysis, and 48% (336) were identified by using transgenic plants (isolation method classified as "overexpression", "knockdown", "knockdown/overexpression", or "others"; Figure 1B).
In the wild type vs. GL mutant analysis, 619 genes were detected as DEGs (differentially expressed genes): 232 DEGs showed a higher expression level in wild type, whereas 387 DEGs showed a higher expression level in the GL mutant.
In planta analyses of the family members, including gene expression, protein localization and mutant analysis, revealed that the members of the two sub-groups have acquired specialized functions, and appear to act in different biological contexts.
Functional molecular analysis of most nematodes, in particular Enoplea, is further hindered by the lack of genetic tools such as mutant analysis or gene-knockdown via RNAi.
Many of these genes were functionally validated using mutant analysis (Morozova et al. 2006; Kong et al. 2010a), but in most cases the molecular and cellular mechanisms by which these genes function have not been determined.
For example, the ability to perform cell-specific knockdown of a particular gene of interest in model organisms (specifically C. elegans in Maher et al. 2013) allows geneticists to study the role of genes in ways that traditional mutant analysis does not allow.
Mutant analysis by targeted gene disruption is a powerful technique for analyzing the function of genes implicated in specific processes (reverse genetics).
