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Gene junction screening suggests that gene order B is shared by all genera in that sub-family, whereas ancestral gene order A is retained in other sub-families of Isididae and in families Primnoidae and Chrysogorgiidae (Brugler and France 2008) (fig. 4).
After eight passages, the replication level of P8 of rVSVR7A with two second-site mutations in the genome (T242 P in N protein and U7-U8 in G-L gene junction) was significantly higher than that of P1.
The promoter proximal gene is transcribed in greatest abundance and each successive downstream gene is synthesized in progressively lower amounts due to attenuation of transcription at each successive gene junction.
However, our findings suggest that this method known as gene junction screening may miss evolutionary reversals.
The amino acid substitution rate in the M COX2e region is ~6× faster than that in the M COX2h+M COX1 gene junction region and >20× faster than that in the F gene junction region (Fig. 4).
And by using gene junction screening, they determine that A. ritteri has the presumed ancestral octocoral mitochondrial gene order.
Similar(44)
Gene overlaps have been found at 11 gene junctions and have involved a total of 55 bp; the longest overlap (14 bp) exists between trnV and trnI.
Gene overlaps have been observed at several gene junctions, varying in size from 20 nucleotides in Dolycoris baccarum to 30 nucleotides in Coptosoma bifaria (Fig. 1, Additional files 1 and 2).
In contrast, reverse transcription-polymerase chain reactions (RT-PCRs) carried out with primers designed to amplify regions spanning gene junctions showed that some mRNAs contained the four genes.
By comparing the genomes of SVCV-C1 with other SVCV strains released in the GenBank, no essential difference was observed in the arrangement of ORF and gene junctions, but there were two differences which can be used as the molecular markers for SVCV-C1.
Three additional short non-coding regions are present at gene junctions.
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