This may be a false negative due to the primers; primer design options for the gene were constrained by high sequence identity to other members of the gene family and so were targeted to a putative 3'UTR of LuBGAL4.
These results also indicate that selection for translational efficiency of highly expressed genes is constrained by the genomic parameters associated with the GC content of the host genome.
Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved.
Notably, the plasticity of the tRNA genes is constrained by the need to maintain proper protein folding (Drummond and Wilke, 2008).
Our extensive analysis of H3K27me3 within the Xic has revealed that a large part of the H3K27me3 hotspot is located between the 3'-ends of two expressed non-coding genes, Tsix and Ftx, suggesting that transcription of both genes is constraining the H3K27 methylation to the region in between.
For our alignment, protein-coding genes were constrained to align by codon and tRNA-coding genes were constrained to align by regions of potential secondary structure [ 38].
Among QTLs with underlying cloned genes, most of the genes were constrained to 0.2 Mb to 7.2 Mb.
Finally, TashAT genes are constrained within, but not between, species and show clear evidence of divergence in the arrangement of conserved functional motifs.
Next, the target genes are constrained by entering the keyword 'purine degradation' in the descriptor textbox in Step 2 (see Figure 6).
