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In contrast, c-Fos, a gene indicative of mechanical stimulation, was upregulated in the marrow cells of mechanically stimulated explants, while the level of activated c-Jun decreased by 25%.
The tfpO gene, indicative for group I pilins, was detected in 48.2% of isolates (Table 3).
Reduced bacterial load in MF pre- incubated with AnxA5 was accompanied by reduced transcriptional activity of the rpo and pyk gene indicative for impaired growth and progeny of chlamydiae (Fig. 6E, n = 3).
Surprisingly, only 47% or 19% of Ade+ transformants with pLAG6 or pLAG10, respectively, contained a CF-linked ADE2 gene indicative of template switching.
Furthermore, in the absence of LSD1, we detected increases in the levels of H3K4me2/me3 and H3K9ac around the TSS of the VAT1L gene, indicative of the loss of LSD1 enzymatic activity and increased transcription.
Chromatin immunoprecipitation (ChIP) and run-on assays demonstrated that knock down of eEF1A1 reduced recruitment of RNAPII throughout the HSP70 gene, indicative of both impaired RNAPII initiation and elongation during heat shock.
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In estimating the physiological condition of the sample, the simultaneous selection of a group of genes indicative of a biological event is a more reliable indicator of that event than the selection of a single pertinent gene.
Neurogenic markers such as nestin, glial fibrillary acidic protein and microtubule associated protein 2 genes were transiently upregulated (a process where cellular components, in this case RNA, is increased in response to external variable) over the first week while genes indicative of osteogenesis remained at its nominal level.
The long-term cellularity and osteogenic differentiation of hMSCs in response to the different compositional ratios were then assessed by quantifying the timed expression of genes indicative of osteogenic differentiation, alkaline phosphatase activity, and DNA content over time.
Only the fully formulated MF59 emulsion induces all the established hallmarks of innate and adaptive immune activation, which includes activation of genes indicative of transendothelial cell migration, strong influx of immune cells into the injection site and their enhanced antigen uptake and transport to the lymph nodes.
We also observed upregulation of genes indicative of differentiation, also bound by Stat3, in ES cells cultured in the absence of LIF.
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