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We associated this gene frame with all protein frames whose encoding genes were not identified.
Previous reports have generated a drug-resistant virus by introducing a Shanghai1 NA segment with a 292K mutation into a Anhui1 gene frame using reverse genetics.
For example, deleting a gene frame will also delete the gene's products, and potentially enzymatic reactions which depend on an enzyme produced by the gene.
In the event any existing functionally unidentified ORF in the PGDB becomes identified as the UDP -d-glucuronate thensporter gene, the corresponding gene frame can easily be re-associated with the UDP -d- glucuronate transporter protein frame in lieu of the unidentified gene frame, without disrupting any other relationships.
To address this, the transport reaction frame was associated with a newly created protein frame named UDP -d- glucuronate transporter that in turn was linked to a newly created gene frame named unidentified gene.
The explicit specification of the intracellular orientation of the catalytic or binding domains of a single polypeptide chain acting as an enzyme might be achieved by creating two separate protein frames, one for each domain, linked to the same gene frame.
Similar(53)
Thus, by targeting a protein coding gene, frame-shift errors caused by insertions or deletions of bases, commonly associated with the 454 pyrosequencing technique [16], can be distinguished and removed.
This frame, unlike other gene frames, does not represent a single gene but a class of unidentified genes.
Deactivation and reiterative labeling experiments were performed similarly by affixing new Gene frames to the slides and repeating the incubation procedure.
The Gene frames were then removed and slides were washed 3 times in 4× SSPE buffer, rinsed again in milli-H2O, and dried under a nitrogen stream.
Some deletions and insertions in the genomes have occurred, resulting in lack of genes, frame shifts, and possible regulatory differences.
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