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The BioMark Dynamic Array microfluidic qPCR system (Fluidigm Corporation, San Francisco, CA) was used for gene expressions analysis of oocytes and cumulus cells.
Fifty genes with the highest and lowest combined score as well as their matching fold changes identified in the gene expressions analysis are shown in Supplementary Table 4.
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We used eXpress (Roberts and Pachter 2013) to perform gene expression analysis.
J.L. performed gene expression analysis.
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Lovén, J. et al. Revisiting global gene expression analysis.
Optimization and validation of gene expression analysis in isolated tissues.
Implement and evaluate one or more gene expression analysis algorithm.
M.A.M. designed and performed experiments including mouse modeling, flow cytometry analysis, and gene expression analysis.
Validation of rat reference genes for improved quantitative gene expression analysis using low density arrays.
Clustering is often one of the first steps in gene expression analysis.
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