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The annotated peak regions were also matched to RNA-Seq gene expression values based on gene name.
Eleven genes representing different functional categories and range of gene expression values based on microarray hybridizations were analyzed using qPCR from cDNA derived from different time point samples.
Six genes representing a range of gene expression values based on microarray hybridizations were analyzed using qPCR from cDNA derived from different time point samples.
Seventeen genes representing different functional categories and the range gene expression values, based on microarray hybridizations were analyzed using qPCR from cDNA derived from stationary phase samples.
The VMus3D, in addition to its accompanying relational database schema also has scripts for transforming and selecting appropriate gene expression values based upon a standard gene set corresponding to the structures within the model for developed adult muscle.
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Then, all probe pairs are used to estimate a gene expression value based on Tukey's Biweight algorithm.
The reliability and reproducibility of the analyses were ensured by the use of duplicates in each experiment, the normalization of all arrays to the median probe intensity level, and the use of normalized intensities of all arrays for the calculation of model-based gene-expression values based on the Perfect Match-only model [ 82].
The reliability and reproducibility of datasets was ensured by the use of duplicate or triplicate hybridization data in each experiment, normalization of all arrays to the median probe intensity and the use of normalized CEL intensities of all arrays for the calculation of model-based gene-expression values based on the Perfect Match-only model [ 42, 43].
Therefore, we quantile transform these cross-platform samples to preserved their relative gene abundances (or gene order) and compute hgu133plus2-like expression values based on a hgu133plus2 reference distribution.
We, therefore, searched our assembled transcriptome for homologs of those genes and analyzed their expression values based on our RNA-seq read count data.
The method of incorporating gene expression values is based on E-Flux reported in the literature [ 46].
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