Sentence examples for gene expression master from inspiring English sources
qRT-PCR was performed using 2 μl of cDNA, TaqMan® Gene Expression Master Mix and the TaqMan® Gene Expression probes of interest (Supplementary Table 4).
TaqMan® gene expression assays for Nfatc1 (Mm00479445_m1), Acp5 (mCG22832), and TaqMan® gene expression master mix (#4369016) were purchased from Applied Biosystems (Foster City, CA).
Real-time PCR analyses were carried out using the Applied Biosystems 7000 sequence detection system and the TaqMan gene expression master mix (Applied Biosystems, Foster City, CA).
Real-time PCR assays were performed in quadruplicate using TaqMan Gene Expression Master Mix (ABI), gene-specific TaqMan TAMRA probes (ABI) and an ABI 7000 sequence detection system.
About 2 µL of cDNA were amplified using the TaqMan® Gene Expression Master Mix and TaqMan® Gene Expression Assay for frataxin (Applied biosystems, catalog n°Hs00175940_m1) in a StepOne real-time PCR.
Quantitative RT-PCR was performed with Power SYBR-Green or Taqman Gene Expression Master Mix (Applied Biosystems) using a 7300 Real-Time PCR system (Applied Biosystems) following the manufacturer's protocols.
Amplification of genomic DNA was performed on an ABI 7900HT Real-time qPCR instrument using Taqman Gene Expression Master Mix (Applied Biosystems).
Relative gene expression was determined on an ABI7900HT quantitative PCR machine (ABI Biosystems) using Taqman Gene Expression Master Mix (Applied Biosystems).
Sequences of pre-made primers and in-house designed primers are shown in supplementary table 3. TaqMan gene expression assay for PKM2 and Cldn5 were used in combination with TaqMan Fast Gene Expression Master Mix Applied Biosciencess) and expression was normalized to endogenous GAPDH also using the Taqman gene expression assay.
Amplification was performed on 20 to 300 ng of cDNA with Taqman gene expression Master Mix Life Technologiess).
Quantitative RT-PCR was performed using TaqMan Gene expression assays (Chk1, Wee1, Caspase 2 and GAPDH) and TaqMan Gene expression Master Mix (Life technologies) as previously described.
