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We also introduced the Thr179Ile (T179I), Ser230Gly (S230G), Arg296Gln (R296Q), Asn308His (N308H), and Cys344Trp (C344W) substitution individually into the human GBGT1 gene expression construct.
The ratios of the cells positive with Forssman antigen versus the cells positive with GFP were calculated, and they were adjusted so that the values become 100 for the cells transfected with the original mouse GBGT1 gene expression construct.
An optimized gene expression construct was designed in order to increase the accumulation of the HIV-1 subtype C p24 protein in Arabidopsis thaliana and carrot (Daucus carota) plants.
qRT-PCR analysis was performed on bacteria having undergone glycerol transformation by the dhaD gene expression construct, which was obtained from K. pneumoniae SRP2.
This gene expression construct allows for ethanol-inducible expression of the β-GLUCURONIDASE (GUS) reporter gene.
AmiR-FT Arabidopsis transgenic plants were transformed with the alc::FTa1 gene expression construct and 19 transformantsnts were selected.
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A. The mBFPvvD8 (D8) or mBFPvvD9 (D9) gene expression constructs driven by RbcS promoter were transiently expressed in tobacco leaves by agroinfiltration, respectively.
DNA from the GBGT1 gene expression constructs and their derivatives was used for transfection.
This work underscores the need for careful design of gene expression constructs.
(c) To investigate temporal changes in gene expression, constructs were compressed at 10% and samples taken for total RNA isolation 0 h, 2 h, or 4 h after compression.
Agroinfiltration of GFP-based gene expression constructs into potato leaf cells is a simple and highly efficient approach to examine protein expression in sub-cellular compartments.
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