Sentence examples for gene expression cassettes from inspiring English sources

Novel linear Ig heavy- and light-chain gene expression cassettes were designed to express Ig VH and VL genes isolated from sorted single B cells as IgG1 antibody without a cloning step.

B. The mBFP or mBFP-mS1C fusion gene expression cassettes driven by RbcS promoter were transiently expressed in tobacco leaves by agroinfiltration, respectively, and the proteins were targeted to cytosol (Cy, pBin-R-Cy-mBFP), apoplast (Se, pBin-R-Se-mBFP), ER (mBFP, pBin-R-Er-mBFP; mBFP-mS1C, pBin-R-Er-mBFP-mS1C), chloroplast (Cp, pBin-R-Cp-mBFP) or mitochondria (Mt, pBin-R-Mt-mBFP), respectively.

Fig. 2 Schematic organization of cocoa gene expression cassettes in each of the expression plasmids.

Promoters, cocoa genes and terminators were fused into cocoa gene expression cassettes using overlap extension PCR (Zhou et al. 2012).

The gene expression cassettes were verified by PCR and the structure of all the cassettes is described in Fig. 2.

The mBFP or mBFP-mS1C fusion gene expression cassettes driven by three different promoters in pBINPLUS vector were shown.

A GFP-2A-Puromycin resistant gene expression cassette was placed after the gRNA cassette both to monitor the transfection efficiency and for selection (Fig. 2A).

EGFP and Cap expression were improved when the chicken HS4 insulator sequence located in the downstream of the foreign gene expression cassette in AcMNPV.

BS was used to modify the polyhedron (polh) promoter, and the chicken HS4 insulator was inserted downstream of the target gene expression cassette.

To improve the safety of MeCP2 gene therapy, the gene expression cassette was modified to include more endogenous regulatory elements believed to modulate MeCP2 expression in vivo.

We also show that homology-directed recombination of the HIVCAR gene expression cassette into the CCR5 locus enhances suppression of replicating virus compared with HIVCAR expression alone.