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Moreover there is a lack of standardization in the experimental designs used for RNA extraction and gene expression assessment (microarrays, RT-qPCR) and for the statistical analyses.
This observation highlights the importance of using multiple complementary analyses for drawing conclusions from gene expression assessment.
Primer sequences used for gene expression assessment are presented in Table 1.
Thus, we collected bone marrow cells from mouse femurs for gene expression assessment.
Gene expression assessment revealed that miR-146a was modulating mRNA levels of IL-6 and MCP-1 in AC16 cells.
Microarray-based gene expression assessment is a very useful method for prediction of diseases, tumor classification and drug responses.
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High-throughput expression profiling technologies have effectively altered the experimental design of gene expression assessments.
ML prepared the manuscript and performed the gene expression assessments and all computational analyses (under the supervision of SK).
Outcome measures were conducted six months post-operative using macroscopic, histology, and selective cartilage protein and gene expression assessments.
High vs. low is a qualitative description deriving from (semi quantitative measurements in the case of q-PCR gene expression assessments.
For gene expression assessments of histone H3 variant genes, all raw values were normalized against two reference genes (ACT1 and αTEBP), using the geometric mean of at least five repeated measurements.
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