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In morphine dependent group PGi nucleus was extracted for gene expression assay 6 h after the last injection of morphine.
PGi was extracted for gene expression assay 24, 48 and 72 h after the last injection of morphine.
The Osteogenic potential of MSCs was evaluated by Alizarin Red staining, ALP activity, calcium content assay and bone related gene expression assay.
Oral treatment of mice with compound 15 robustly and dose-dependently inhibited IL-17A production in an IL23-induced gene expression assay.
Taqman gene expression assay for HVCN1 was purchased from Applied Biosystems (Assay ID: Hs00260697_m1).
TaqMan gene expression assay primers were used for mouse megalin, Acox and GADPH Applied Biosystemss).
The Drosophila GAPDH TaqMan gene expression assay was used as the endogenous control (assay ID: Dm01841185_m1).
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The ER-CALUX is an "estrogen-receptor mediated, chemical-activated luciferase reporter gene-expression assay" based on human U2OS cells with an exogenous hERα receptor [39].
Validation of the downregulation of PTK9 was performed using a TaqMan® Gene Expression Assay (Assay ID: Hs00702289_s1).
Taqman(R) Gene Expression Assay (Applied Biosystems) was purchased for Mash1 with Assay ID Hs00269932-m1.
mRNA expression was measured by real-time PCR using TaqMan probe (TaqMan Gene Expression Assay): VGF (Assay ID Details: Hs00705044_s1).
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