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Total RNA was then isolated and gene expression analyzed using Clontech Atlas Rat 1.2 Toxicology arrays.
Figure S4 shows clusters of gene expression analyzed using DAVID.
RNA samples were prepared and gene expression analyzed as described previously [7].
Differential gene expression, analyzed with Illumina Beadstation, was used to detect intracellular signaling resulting in changed transcription, compared to control cells (n = 2 in each group).
Total RNA was extracted from each sample and gene expression analyzed by microarray.
All reactions were run in triplicate from at least three independent experiments and gene expression analyzed using delta Ct values.
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During a typical day I spend time doing "gene expression," analyzing what distinguishes the genetic signature of a normal person from a diseased person.
We therefore tested whether ERK signaling in T cells also induces hexokinase gene expression, analyzing the same mRNA samples that we used previously to study glutaminase expression.
The microarray technology facilitates large-scale surveys of gene expression data for whole-genome mapping and gene expression analyzing under various conditions [ 4].
These morphological and anatomical data were supported by our gene expression analyzes.
Furthermore, the low accuracies suggest that there are no dominant predictors within the gene expression dataset analyzed in this study.
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