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Functional high-throughput screening of effector gene elicitation of the HR in plants was performed using a Potato virus X (PVX) agroinfection assay optimized for Solanum [13].
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Considering the slower elicitation of HLR at 33°C (Figure 3B), signaling involved the HMGR gene induction may not play a role in the elicitation of HLR at 33°C.
For isolation of CD4+ cells for gene expression analysis, elicitation was performed on a 2-cm shaved area on the back torso of the mouse.
In addition, many of these genes function during the elicitation of inflammation, supporting our assumption that the genes augmented in both models may function during the effector phase.
Mechanistically, specific resistance relies on the recognition of avr pathogen factors by plant R gene products and the elicitation of local defense responses, often associated with a rapid programmed cell death, called the hypersensitive response (HR).
Since StWhy1 has been shown to act as a transcriptional activator for the PR-10a nuclear gene in tubers following elicitation, it is plausible that AtWhy2 could take part in the regulation of transcription in mitochondria [ 4, 6].
StWhy1, the prototypical Whirly from Solanum tuberosum, has been characterized as a transcriptional activator of the pathogenesis-related gene PR-10a following elicitation or wounding of potato tubers [ 4- 6].
In the anoxygenic photosynthetic bacterium Rhodobacter sphaeroides, a σE factor is responsible for the elicitation of the gene expression response to O2 (Anthony et al., 2005).
The trial used DNA prime and rAd5 vector boosts with multiple antigens (HIV-1 modified env genes from clades A, B, and C, and gag and pol genes from clade B) for elicitation of both antibody and T-cell responses and was performed on subjects without pre-existing antibodies against rAd5.
Why these low abundant isoforms of photosynthetic genes are up-regulated after elicitation is unknown.
However, semi-quantitative RT-PCR could not detect transcripts of these genes on day 5 after elicitation.
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