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The passaged viruses maintained the eGFP gene, demonstrating the stability of the reporter virus.
All passaged viruses stably maintained the luciferase gene, demonstrating the stability of the reporter virus.
Similar results were observed for the expression of both GFP and the T. gondii HXGPRT gene, demonstrating the generality of this effect.
The present approach proved to be capable of detecting the wild target DNA down to a detection limit of 1.0 × 10−14 M in a wide target concentration range and identifying −28 site (A to G) of the β-thalassemia gene, demonstrating that this scheme offers a highly sensitive and specific approach for point mutation detection.
Prerequisites for gene therapy include finding the best delivery system (often a virus, typically referred to as a viral vector) for the gene, demonstrating that the transferred gene can express itself in the host cell, and establishing that the procedure is safe.
Chen et al. (2008) cloned the S5 gene, demonstrating that S5 encodes an aspartic protease conditioning embryo-sac fertility, the allelic difference being very small at the sequence level.
Similar(32)
Transgenic mice with a K15 promoter driving the expression of a reporter gene demonstrated that the K15 positive epidermal cells have stem cell characteristics25,26.
The complex case of the OsNAC6 gene demonstrates that it will probably be difficult to use this category of genes to improve stress tolerance (Nakashima et al. 2007).
For the three genetic alterations (mutations, CNGs or both) each gene demonstrated a distinct pattern.
Analysis of the cytochrome b gene demonstrated that the captured bats belonged to the species M. myotis (data not shown).
The gene demonstrated was blaCMY2.
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