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The main active component of these products is gene constructions (nucleic acids).
Gene constructions consist of a therapeutic gene (cDNA or RNA) and its intracellular delivery system (vector).
For that, ordinary materials as the scaffolds are combined with cells, growth factors, or gene constructions encoding them.
Gene constructions were transformed into Agrobacterium strain GV3101 by the freeze thaw method, and the transformants were grown overnight in Luria-Bertani (LB) medium.
Similarly, we investigated the feasibility of direct in vitro recombination into DEST-BAC using the attL flanking B5R and A33R gene constructions (schematic diagrams shown in Figure 6A).
It is important that, even in such a case, positive results were obtained, which proved the supreme importance of gene constructions in gene-activated materials.
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This in vitro gene construction might also simulate the mosaic nature of novel genes.
Discussed here are basic structure of such reporters, design considerations, reporter gene construction, cellular and in vitro characterization.
At the same time, I had a great opportunity to learn much about the molecular aspects of the work, from gene construction to plant transformation and screening.
Our results demonstrate that microbial community genetic diversity can serve as a source for novel gene construction during in vitro manipulation.
To achieve the objectives, we produced transgenic rice plants containing a chimeric gene construction with the potential promoter region of SH4 fused with the GUS gene, and examined the expression pattern of GUS activity.
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