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Thus, the burgeoning field of synthetic biology has great promise to benefit from gene design tools such as Gene Composer.
The native gene sequences were divided into Oligo-sets using the manual Oligo-set creation feature in Gene Composer.
At this point, each target was moved into the Gene Design module (Protein-to-DNA) of Gene Composer™.
For this reason, we have intentionally enabled Gene Composer to accept numerous user defined settings for gene design parameters.
The template sequence can either be a native cDNA sequence or a codon engineered synthetic DNA sequence crafted by the Gene Design Module of Gene Composer.
Based on these user defined parameters, Gene Composer generates thousands of possible new synonymous gene sequences for any given protein sequence.
In this report, we compare heterologous protein expression levels from native sequences to that of codon engineered synthetic gene constructs designed by Gene Composer.
When applied to a gene sequence, each of the available codon engineering strategies described below are tracked in the Gene Composer database as a sequence of engineering events.
These results clearly show that proteins expressed from a synthetic Gene Composer engineered gene can be successfully used in protein crystallization experiments.
Sequence verified whole gene synthesis products of native and Gene Composer engineered versions of P38α and NS5B were used as PCR templates for cloning into vector pEU-E01.
To demonstrate the utility of this approach, we have used Gene Composer to design protein constructs, as well as the synthetic gene sequences to express those proteins.
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