Sentence examples for gene collections from inspiring English sources
Because SCOOP minimises costs for cloning and expression screening, it constitutes an interesting alternative for establishing expression of large gene collections.
We have designed and evaluated a novel strategy for screening large gene collections available as GATEWAY-adapted ORFeomes for soluble recombinant overexpression in Escherichia coli, called "Screening Colonies of ORFeome Pools" (SCOOP).
Chi-square tests were performed to determine differences in frequency distribution for given intron sizes between the different types of gene collections.
MSigDB gene collections overlapping with EWS-FLI1 up-regulated genes showed relatively strong clustering due to the similarity of the respective gene sets, while down-regulated genes showed only little overlap.
The similarity among the significant MSigDB gene collections was further explored by identifying the ten most significant gene sets from c2 and c3 by their combined z-score (see Methods), and their proximity in gene space was determined using gDist.
Extended transcript clusters are based on cDNA evidence and include other human mRNA and EST sequences, ENSEMBL gene collections, synthetically mapped mRNAs from mouse and rat, mitoMap mitochondrial genes, microRNA registry genes, vegaGene and vegaPseudoGene records.
Axolotl genes were cloned using gene-specific primers designed using sequences collected from the Ambystoma Gene Collection (Smith et al., 2005).
The main purpose of gene collection from metatranscriptomic data is, as stated above, to collect as many genes as possible with functional annotations.
We discuss two downstream applications of this gene collection: production of soluble HLA molecules and discovery of new epitopes.
From a large gene collection we could, without expensive multi-well based cloning and expression screening, determine which targets were suitable for large-scale expression and purification.
A comprehensive gene collection for S. oneidensis was constructed using the lambda recombinase (Gateway) cloning system.
