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The analysis of effector gene sequences in HERB-1 strains should reveal the effector repertoire prior to its disruption by the selective forces imposed by resistance gene breeding.
That HERB-1 has so far not been found in any modern strain may point to its extinction after the 19th century pandemic, possibly associated with the onset of resistance gene breeding in the 20th century.
Therefore, if the major selection pressure that led to the replacement of HERB-1 by US-1 was the introduction of resistance gene breeding, greater variation at effector genes in polyploid US-1 strains could have contributed to the replacement of HERB-1 soon after R genes from S. demissum and other wild species had been introduced into modern potato germplasm.
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If there are interactions among the objective genes, breeding strategy must be addressed by the incorporation of the epistasis [51], [52].
Molecular markers tightly linked to the identified BPH resistance genes are playing the important role in MAS based gene pyramiding breeding programs for the rapid selection of genotypes with targeted gene (Collard et al., 2005; Hayashi et al., 2010).
These results provide new information that the identified WRKY genes from cold-resistant bermudagrass may serve as a target gene for breeding new varieties in future.
Through the backcrossing and gene pyramiding breeding coupled with molecular marker-assisted selection, a new P/TGMS line Hua1228S carrying Pi2, Xa7, and tms5 was developed.
It is critical to understand that the value of a resistance gene in breeding programs could be compromised if it dramatically affects plant development and morphology.
One important step for studies supporting its conservation is the characterization of its genetic diversity and structure, gene flow, breeding ratio of populations, and mating system.
Strong associations were found for novel SNPs linked with known bacterial blight resistance Xa genes, from which high utility markers for tracking and selection of resistance genes in breeding programs were designed.
Many efforts seek to use genomics-based strategies to identify genes controlling flavor, and then designing durable molecular markers to follow these genes in breeding populations.
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