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The photoluminescent property, low cytotoxicity, biodegradation, good gene binding and protection ability and high genes delivery efficiency make POCG-PEI highly competitive as a non-virus vector for genes delivery and real-time bioimaging applications.
Preliminary studies on gene binding, cell toxicity, and cell uptaking in vitro were carried out.
The result demonstrated that arginine-modified HAP had good biocompatibility and gene binding property.
Their gene binding and condensation capacities as well as their in vitro and in vivo transfection properties have been reported in recently ample literatures.
The last one is the human microRNA target gene binding site information from Sanger's miRBase (http://microrna.sanger.ac.uk/).sanger.ac.uk/
However, methods for predicting miRNA targets have proved to be a major barrier in the field, mainly due to the incomplete understanding of miRNA target gene binding interaction.
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First is the physical network that includes PPIs and PDIs at the factor-gene binding level.
The second one, called the TF-gene binding network, was constructed using the TF-gene binding pairs.
Two nodes connected by a directed edge in the TF-gene binding network represent a TF-gene binding pair with literature supports under the specified experimental condition.
And we have expanded the number of functional TF-gene binding pairs by about 6 folds.
All in all, our method can extract functional TF-gene binding pairs with better expression coherence.
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