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The phosphinothricin acetyltransferase gene (bar) driven by CaMV35S promoter in pLSP15 and pLSP20, and ubiquitin promoter in pLSP13 and pLSP23 were used as the plant selectable markers.
Wan et al. (2014) reported development of a new elite restorer line possessing tolerance to BPH, stem borer, leaf folder and herbicide through pyramiding Bph14, Bph15, Cry1C, and glufosinate-resistance gene bar.
In addition, the T-DNA region of the binary vector (used for rice transformation) contained a selectable marker gene bar for the herbicide bialaphos resistance (Fig. 1A).
Several genes, including Atriplex hortensis betaine aldehyde dehydrogenase (badh) involved in the accumulation of glycine betaine giving tolerance to salt stress [24], isoflavone synthase (ifs) from Medicago truncatula that a key enzyme in the flavonoids/isoflavonoids pathway [25], a phosphinothricin resistance gene (bar) and two reporter genes, gus and gfp, were chosen for gene stacking.
To overcome repression, we deleted the gene creA by using the gene bar as the marker.
The second fragment was the selectable marker gene bar, amplified from vector pTJK1 (Jones et al. 2007).
Similar(45)
All the transformant lines obtained in the present investigation displayed functional activity of selectable marker genes (bar and nptII genes).
In case of M-type genes, barring OsMADS90, 91 and 96, all other genes exhibited similar groupings as in the MADS-domain-specific phylogenetic analysis.
An inverse relationship (Pearson correlation between −0.5 to −0.9) for 16 families covering 135 genes (barring 1 2 outliers in each) was found.
In addition, nearly half of the other T4 signature genes (indicated by the black font directly above coloured genes bars in Figure 4) are shared by all 5 T4-related Acinetobacter phages, including the gene topology.
All of the Core genes described by Petrov et al. exist in all 5 T4-related Acinetobacter phages and are indicated by the red font directly above the coloured genes bars in Figure 4.
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