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The coding potential of a gene assembly is the sum of scores of assembled exons: P g) = P e1')+...+ P e q' ).
Figure 4 illustrates how the assembled T. cruzi chromosomes can help clarify gene assembly issues.
Using this technology, DNA oligo synthesis and gene assembly steps occur right on silicon.
Multi-step reaction schemes utilizing various genetic engineering techniques described above were employed for solid-phase gene assembly.
A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was used for generation of standard RNAs.
A DNA fragment was amplified for mimicking the complementary RNA by PCR-based gene assembly, and was used for generation of standard RNA templates.
For gene assembly, oligonucleotides were designed by Thermodynamically Balanced Inside Out (TBIO) method using the published synthetic codon optimized hIFNα2b ORF for high expression in E. coli.
The gene assembly was based on a polymerase chain reaction, in which the polymerase extends DNA fragments from a pool of overlapping oligonucleotides.
The structural analysis of a number of recombinants and the derivation of secondary recombination products define some of the basic features of the mechanism of immunoglobulin gene assembly.
We applied a DNA assembly protocol in B. subtilis, named ordered gene assembly in B. subtilis (OGAB) method, to incorporate those three gene blocks into a one-unit plasmid via one ligation-reaction.
Gene assembly and amplification were performed in two separate PCR reactions (Thermo Hybaid Sprint).
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