Functional marker gene analyses were performed on either an Applied Biosystems Step One or an MJ Research quantitative PCR system.
Six of the strains were identified as Staphylococcus haemolyticus and one strain as Staphylococcus arlettae by 16S ribosomal gene analyses.
Hence, both whole genome and individual gene analyses could be made in a comprehensive manner across many species, as first proposed and illustrated in the grasses.
However, only data obtained from the Illumina HiSeq II was used for pathogenic gene analyses due to its high sequence fidelity.
Reporter gene analyses indicated induction of the SOS response for some of the derivatives, suggesting interference with DNA structure or metabolism.
As a major result of this study, a very useful tool is now available for future functional gene analyses as well as genetic engineering approaches.
We further identified the Kapoposang sponge specimen used in this work as Rhabdastrella sp. based on the integrated morphological, histological, and cytochrome oxidase subunit I (COI) gene analyses.
This additional control would allow the analysis of an absolute change in gene expression, in addition to the relative changes that current gene analyses can detect.
The strongest phylogenetic support came from single gene analyses of the 123cdp alignments.
