Sentence examples for gene amount from inspiring English sources

Briefly, the target gene amount was normalized to the endogenous reference (HPRT) and then compared against a calibrator (sample with the lowest expression, namely, sham-operated animals), using the formula 2−DDCT.

Gene amount was assessed by quantitative real-time RT-PCR, using the QuantiTec SYBR Green PCR kit (Qiagen), 2 µL of total cDNA, and 500 nM of each primer.

The target gene amount was divided by the housekeeping gene amount to obtain a normalised target value.

Results presented in histogram are the normalized target gene amount relative to control 2−ΔΔCT.

The amount of specific gene was subsequently divided by the β2-microglobulin gene amount to obtain normalized specific gene value.

The target DNA amount was divided by the housekeeping gene amount to obtain a normalized target value.

A standard curve was derived from serial dilutions to quantify the copy numbers of target mRNAs, and gene amounts were normalized to β-actin levels.

However, these genes amount to less than 4% of the non-conserved gene pairs.

The most significant term is molecular function (MF): zinc ion binding (FDR = 1.6-E4), where C2H2 ZNF genes amount over 60% of the total hits.

The computer matched it with interleukin 3 of mice, even though the match between the DNA subunits of the two species' genes amounted to only 47percentt.

Using strict rules for homology determination, the subset of grape specific genes amounted to 16,859 (Figure 2).