Gender of offspring can be assigned at random to create two sexual populations, but it makes no difference to the computation since the two gender pools are essentially the same in their composition.
The QC samples used in this study were QC1, which was normal human plasma (mixed gender, pooled; PLH-123-F; Sera Laboratories International Ltd ,Haywards Heath, West Sussex, UK) and QC2, which was normal pooled horse plasma (S-121-F; Sera Laboratories International).
Equal volumes of individual saliva samples were pooled to construct different age and gender pools that were as homogeneous as possible to reduce the individual variance, and the protein amount was measured using a BCA protein analysis kit (Pierce, Rockford, IL).
However, Joinson and Nettle found a trend of increased sensation seeking in both genders pooled, while we only found the effect in males.
For brain and liver, an additional gender-pooled de novo sequence read assembly was performed.
Moreover, there was a strong correlation between MUAC and BMI (r = 0.87, P < 0.001) for both genders pooled.
Mixed effects model was used to establish unconditional and conditional standards in z-scores and percentiles for both genders pooled and for each gender separately.
For both genders pooled TSF correlated well with fat mass (r = 0.68, P < 0.001), and AMC correlated well with fat-free mass (r = 0.68, P < 0.001), at baseline.
The unconditional standard for both genders pooled is shown in Fig. 1 in terms of percentiles, after back-transforming the AC and GA values to their original scales.
The data of all 4 × 8 animals of each gender were pooled, and the 'true' gender differences in 18F-FDG uptake were determined.
