Radioiodination with the positron-emitting isotope 124I was done by the Iodo-Gen method as described [5].
14C5 chAb and its derivatives were labeled with 125I or 131I (PerkinElmer, Zaventem, Belgium) by incubating 10 min at room temperature using the Iodo-Gen method [27].
The tracer was made by labeling Tyr (25 37) amide α-CGRP (Multiple Peptide Systems, San Diego, CA) by the Iodo-Gen method (Pierce, Rockford, IL).
In vitro stability, specificity, and affinity of radioiodinated chAb and fragments (Iodo-Gen method) were examined on high-expressing αvβ5 A549 lung tumor cells.
L19-SIP was radioiodinated by using a modified version of the IODO-GEN method.
L19-SIP was labelled according to the IODO-GEN method as described by Visser et al (2001).
Pruszynski and coworkers reported the radio-iodination of a cysteine-tagged anti-HER2 nanobody (5F7GGC) with 125/131I, comparing iodination through the conventional IODO-GEN method with labeling via the residualizing agent Nϵ- 3-[131I]iodobenzoyl -Lys5-Nα-maleimido-Gly1-GEEEK (IB-Mal-DGEEEK) [76].
As mentioned in the Materials and Methods section, L19-SIP antibody was labelled following the IODO-GEN method with both I and I. Preliminary studies showed that the protein could be labelled using the methods described above and a plateau of labelling was found at 4 min.
Once cultured, M. tuberculosis and NTM were differentiated using the Gen-Probe® method (Gen-Probe; San Diego, CA, USA) [ 14].
Although linkage relationships can be determined using other next-gen methods, it requires sophisticated computational methods (Snyder et al. 2015).
In addition, the linkage relationships of single-nucleotide polymorphisms (SNPs) in complex genomes generally requires longer sequence reads than are obtained with common next-gen methods.
